What happened when the second vial of God's wrath was poured out (Rev 16)?

These are they which came out of great tribulation, and have washed their robes, and made them white in the blood of the Lamb. Revelation 7:14

The second angel poured out his vial upon the sea; and it became as the blood of a dead man: and every living soul died in the sea. Revelation 16:3

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